Factors Affecting Recombinant Protein Yields in Insect Cell/Baculovirus Expression System

Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System

Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...

Factors affecting the expression of recombinant glycoproteins.

N-glycosylation is both species and tissue specific with a series of membrane bound glycosidases and glycosyltransferases modifying the oligosaccharide as it moves through the endoplasmic reticulum (ER) and Golgi. Each of these individual enzymatic reactions may not go to completion; therefore giving rise to glycoforms of the polypeptide. Glycosylation patterns of recombinant proteins are relev...

Expression of a New Recombinant Collagenase Protein of Lucilia Sericata in SF9 Insect Cell as a Potential for Wound Healing

Background: Today, the use of maggot therapy has become widespread due to the increase in chronic ulcers in the world. The recombinant production of secreted enzymes from these larvae is a novel non-invasive method for the treatment of chronic ulcers. Lucilia sericata (L. sericata) collagenase (MMP-1) has been expressed in insect cells. Collagenase is an enzyme that is widely used in clinical t...

Advances and challenges in recombinant protein expression in Escherichia coli

cloning and expression of simian rotavirus spike protein (vp4) in insect cells by baculovirus expression system

background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...